6L69
Crystal structure of CYP154C2 from Streptomyces avermitilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2019-03-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.780, 53.390, 98.581 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 33.890 - 1.500 |
| R-factor | 0.1691 |
| Rwork | 0.168 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.988 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.091 | 0.616 |
| Number of reflections | 112411 | 16190 |
| <I/σ(I)> | 13.2 | 3.2 |
| Completeness [%] | 99.5 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris (pH 6.5), 28% PEGMME 2000 |
