6L5T
The crystal structure of SADS-CoV Papain Like protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-01-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.231, 68.511, 104.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.260 - 1.720 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.21160 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.510 | 1.720 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.116 | 1.636 |
| Rmeas | 0.121 | 1.705 |
| Rpim | 0.034 | 0.663 |
| Number of reflections | 26961 | 1362 |
| <I/σ(I)> | 16.7 | 1.7 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 12.8 | 12.5 |
| CC(1/2) | 0.999 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 0.1M MES (PH6.5), 0.1M CaCl2, 16% PEG 20000 |
