6L5Q

crystal structure of GgCGT in complex with UDP-Gal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	85.827, 139.278, 73.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.911 - 2.894
R-factor	0.2285
Rwork	0.226
R-free	0.27650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2acw
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.950
High resolution limit [Å]	2.894	7.850	2.900
Rmerge	0.175	0.073	0.737
Rmeas	0.185	0.078	0.788
Rpim	0.058	0.025	0.267
Number of reflections	9820	568	387
<I/σ(I)>	3.3
Completeness [%]	96.2	100	78
Redundancy	9.6	9.2	7.5
CC(1/2)		0.997	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium acetate, 0.1 M Tris, pH 8.5, 25% w/v Polyethylene glycol 3350