6L5P

crystal structure of GgCGT in complex with UDP-Glu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	87.178, 139.468, 73.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.448 - 2.603
R-factor	0.2387
Rwork	0.237
R-free	0.27530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2acw
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.073	0.029	0.913
Rmeas	0.080	0.032	0.996
Rpim	0.032	0.013	0.392
Total number of observations	85426
Number of reflections	13994	768	694
<I/σ(I)>	5.5
Completeness [%]	98.9	98.1	97.5
Redundancy	6.1	5.4	6.3
CC(1/2)		0.998	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium acetate, 0.1 M Tris, pH 8.5, 25% w/v Polyethylene glycol 3350