6L5L

Crystal structure of human DEAD-box RNA helicase DDX21 at apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	146.170, 46.630, 58.490
Unit cell angles	90.00, 101.16, 90.00

Refinement procedure

Resolution	34.120 - 3.100
R-factor	0.194
Rwork	0.191
R-free	0.24100
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.703	36.151	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge		0.052	0.325
Rmeas	0.140	0.060	0.370
Rpim	0.065	0.030	0.172
Total number of observations	26341	877	3993
Number of reflections	6479	227	952
<I/σ(I)>	9.8	18.1	4.5
Completeness [%]	90.5	92.7	92.1
Redundancy	4.1	3.9	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% pET3350, 0.2M Na2HPO4