6L5L
Crystal structure of human DEAD-box RNA helicase DDX21 at apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.170, 46.630, 58.490 |
| Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
| Resolution | 34.120 - 3.100 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.703 | 36.151 | 3.270 |
| High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
| Rmerge | 0.052 | 0.325 | |
| Rmeas | 0.140 | 0.060 | 0.370 |
| Rpim | 0.065 | 0.030 | 0.172 |
| Total number of observations | 26341 | 877 | 3993 |
| Number of reflections | 6479 | 227 | 952 |
| <I/σ(I)> | 9.8 | 18.1 | 4.5 |
| Completeness [%] | 90.5 | 92.7 | 92.1 |
| Redundancy | 4.1 | 3.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% pET3350, 0.2M Na2HPO4 |
