6L5K

ARF5 Aux/IAA17 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97960
Spacegroup name	P 41 21 2
Unit cell lengths	79.894, 79.894, 116.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.730 - 2.910
R-factor	0.2302
Rwork	0.226
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4chk 2muk
RMSD bond length	0.011
RMSD bond angle	1.158
Data scaling software	HKL-2000
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.010
High resolution limit [Å]	2.900	2.900
Number of reflections	8612	8594
<I/σ(I)>	43.2
Completeness [%]	98.0
Redundancy	8.9	4
CC(1/2)	0.989	0.438

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	8.5	293	Ammonium sulfate