6L5J

Crystal structure of human rootletin 1108-1317

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97942
Spacegroup name	P 1
Unit cell lengths	60.389, 67.997, 79.772
Unit cell angles	92.62, 108.50, 113.84

Refinement procedure

Resolution	47.473 - 2.770
R-factor	0.2833
Rwork	0.283
R-free	0.28990
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.17-3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.820
High resolution limit [Å]	2.770	7.510	2.770
Rmerge	0.100	0.058	0.454
Rmeas	0.119	0.069	0.544
Rpim	0.064	0.038	0.296
Number of reflections	25990	1324	1221
<I/σ(I)>	4.8
Completeness [%]	95.1	97.1	90.3
Redundancy	3.5	3.4	3.2
CC(1/2)		0.997	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	278	0.1 M Tris pH 7.5, 4% PEG 6000