6L5B

The structure of the UdgX mutant H109E at a post-excision state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-14
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	38.736, 50.923, 40.285
Unit cell angles	90.00, 91.31, 90.00

Refinement procedure

Resolution	21.275 - 2.000
R-factor	0.173349796812
Rwork	0.171
R-free	0.21233
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6io9
RMSD bond length	0.009
RMSD bond angle	1.073
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.275	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.065	0.233
Number of reflections	10298	1484
<I/σ(I)>	10.1	3.4
Completeness [%]	96.8	95.2
Redundancy	2.5	1.8
CC(1/2)	0.998	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	2.15M sodium malonate pH 7.0 and 3.8% MPD