6L58
Cu(II) loaded Tegillarca granosa M-ferritin soaked with Fe(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.73890 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 217.288, 217.288, 134.083 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.399 - 3.903 |
| R-factor | 0.191065939557 |
| Rwork | 0.187 |
| R-free | 0.26354 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a9k |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.503 |
| Data reduction software | xia2 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.970 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.167 | 0.523 |
| Number of reflections | 10532 | 412 |
| <I/σ(I)> | 10.357 | 1.8 |
| Completeness [%] | 94.0 | 73.4 |
| Redundancy | 3.5 | 3.1 |
| CC(1/2) | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M ammonium acetate, 0.1M TRIS hydrochloride pH 7.5, a precipitant solution of 30% v/v 2-Propanol |
