6L4R

Crystal structure of Enterovirus D68 RdRp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97775
Spacegroup name	P 41 21 2
Unit cell lengths	59.835, 59.835, 358.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.960 - 2.147
R-factor	0.199
Rwork	0.197
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xe0
RMSD bond length	0.007
RMSD bond angle	0.816
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.962	2.210
High resolution limit [Å]	2.147	2.150
Rmerge	0.131	0.638
Number of reflections	37072	3002
<I/σ(I)>	33.5
Completeness [%]	99.6
Redundancy	25.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	0.1M Tris,PH8.0, 3.5M NH4Cl,10 mM Chromic chloride hexahydrate