6L4L
Crystal structure of S. aureus CntK in inactive state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2017-12-28 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.748, 60.293, 76.991 |
| Unit cell angles | 90.00, 116.91, 90.00 |
Refinement procedure
| Resolution | 30.160 - 2.000 |
| R-factor | 0.2022 |
| Rwork | 0.201 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jis |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.286 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.501 | |
| Rmeas | 0.072 | |
| Number of reflections | 19154 | 1897 |
| <I/σ(I)> | 20.646 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.05 M HEPES pH7.5, 26% PEG 400 |
