6L4K
Human serum albumin-Palmitic acid-Cu compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2018-11-25 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.356, 93.523, 96.359 |
| Unit cell angles | 74.89, 89.68, 80.04 |
Refinement procedure
| Resolution | 46.530 - 2.090 |
| R-factor | 0.2385 |
| Rwork | 0.236 |
| R-free | 0.28980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e7h |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.731 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 2.139 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.051 | 0.056 |
| Number of reflections | 63319 | 3977 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | PEG3350, 50 mM potassium phosphate (pH 7.5), 5% glycerol, and 4% DMSO. |
