6L4B

Crystal structure of human WT NDRG3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	173.341, 100.150, 110.744
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	46.671 - 2.200
Rwork	0.168
R-free	0.18480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xmq
RMSD bond length	0.002
RMSD bond angle	1.143
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.101	0.641
Number of reflections	95796	4774
<I/σ(I)>	15.9
Completeness [%]	99.7
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	200 mM sodium citrate tribasic dehydrate, 20% PEG 3350