6L3Q

Crystal structure of Chaetomium thermophilum Utp15 C terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	106.188, 106.188, 59.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.286 - 2.702
R-factor	0.2028
Rwork	0.199
R-free	0.24070
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.062
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.067
Number of reflections	20360	512
<I/σ(I)>	35.93	17.95
Completeness [%]	99.9
Redundancy	21.3
CC(1/2)		0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293.15	1.6M MgSO4, 0.1M Mes