6L3F
The structure of UTP:RNA uridylyltransferase 1 (URT1) in in Arabidopsis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.320, 65.086, 66.115 |
| Unit cell angles | 90.00, 121.15, 90.00 |
Refinement procedure
| Resolution | 35.104 - 1.975 |
| R-factor | 0.1732 |
| Rwork | 0.172 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w0n |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.104 | 2.040 |
| High resolution limit [Å] | 1.975 | 1.975 |
| Rmerge | 0.070 | 0.421 |
| Number of reflections | 31325 | 31325 |
| <I/σ(I)> | 19.91 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES pH 7.5,10% PEG6000,5% MPD |
