6L34

Crystal structure of the HMG domain of human FACT complex subunit SSRP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 62
Unit cell lengths	64.985, 64.985, 55.074
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.362 - 1.996
R-factor	0.1766
Rwork	0.175
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fgh
RMSD bond length	0.007
RMSD bond angle	0.729
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.362	2.068
High resolution limit [Å]	1.996	1.997
Rmerge	0.078	0.327
Number of reflections	9083	909
<I/σ(I)>	27.4
Completeness [%]	99.8
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2M DL-malic acid PH7.0, 0.1M BIS-TRIS propane pH7.0.