6L29

The structure of the MazF-mt1 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-30
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	35.644, 51.872, 129.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.970 - 2.300
R-factor	0.184667275305
Rwork	0.182
R-free	0.23201
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kys
RMSD bond length	0.004
RMSD bond angle	0.696
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.972	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.092	0.264
Number of reflections	10906	1525
<I/σ(I)>	10.8	3.7
Completeness [%]	96.9	94.9
Redundancy	2.9	2.7
CC(1/2)	0.994	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	20% PEG 3350, 0.1 M NaOAc pH 5.0, and 0.1 M NaCl