6L27
X-ray crystal structure of the mutant green fluorescent protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.850 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.875, 62.561, 68.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.486 - 0.770 |
| R-factor | 0.1186 |
| Rwork | 0.118 |
| R-free | 0.12390 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.486 | 0.800 |
| High resolution limit [Å] | 0.770 | 0.770 |
| Rmerge | 0.067 | 0.740 |
| Number of reflections | 258331 | 25594 |
| <I/σ(I)> | 61.45 | 2.78 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES-NaOD (pD 7.0), 5.0 % w/v PEG 2000 and 50 mM NDSB |
