6L26

Neutron crystal structure of the mutant green fluorescent protein (EGFP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	NUCLEAR REACTOR
Source details	FRM II BEAMLINE BIODIFF
Synchrotron site	FRM II
Beamline	BIODIFF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-11-14
Detector	BIODIFF
Wavelength(s)	2.668
Spacegroup name	P 21 21 21
Unit cell lengths	50.857, 62.160, 69.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.764 - 1.444
R-factor	0.1696
Rwork	0.168
R-free	0.20650
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2wur
RMSD bond length	0.012
RMSD bond angle	1.389
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.800	1.490
High resolution limit [Å]	1.450	1.450
Rmerge	0.098	0.177
Number of reflections	90310	25594
<I/σ(I)>	7.9	1.9
Completeness [%]	88.9	73.8
Redundancy	2.5	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES-NaOD (pD 7.0), 5.0 % w/v PEG 2000 and 50 mM NDSB