6L1X
Quinol-dependent nitric oxide reductase (qNOR) from Neisseria meningitidis in the monomeric oxidized state with zinc complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.120, 116.680, 123.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.920 - 3.150 |
| R-factor | 0.25313 |
| Rwork | 0.250 |
| R-free | 0.30253 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.999 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.920 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.113 | 0.556 |
| Rmeas | 0.141 | 0.700 |
| Rpim | 0.064 | 0.420 |
| Number of reflections | 19034 | 3849 |
| <I/σ(I)> | 6.5 | 2.2 |
| Completeness [%] | 83.3 | 84.5 |
| Redundancy | 4.1 | 4 |
| CC(1/2) | 0.997 | 0.284 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 50 mM HEPES pH8 26% PEG 600 (v/v) 0.5 mM zinc sulphate |
