6L1W

Zinc-finger Antiviral Protein (ZAP) bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	34.450, 80.548, 84.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.274 - 2.194
R-factor	0.2076
Rwork	0.206
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u9g
RMSD bond length	0.005
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.190	2.190
Rmerge		1.286
Number of reflections	12471	616
<I/σ(I)>	25.3
Completeness [%]	99.9
Redundancy	12.2
CC(1/2)	0.999	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris, pH 7.5, 0.25 M MgCl2, 18% PEG3350