6L1V
Domain-swapped Alcaligenes xylosoxidans azurin dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-01 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.451, 167.809, 116.988 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.170 - 2.250 |
R-factor | 0.20414 |
Rwork | 0.203 |
R-free | 0.23021 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rkr |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.170 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.079 | 1.145 |
Number of reflections | 50385 | 4598 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 99.9 | |
Redundancy | 6.8 | |
CC(1/2) | 0.739 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES-NaOH, 25% (w/v) PEG 3000 |