6L1R

Crystal structure of N-terminal domain of human SSRP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002+
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-23
Detector	AGILENT EOS CCD
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	37.825, 37.825, 68.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.417 - 1.798
R-factor	0.1764664673
Rwork	0.173
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4khb
RMSD bond length	0.008
RMSD bond angle	1.057
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.417	1.880
High resolution limit [Å]	1.798	1.800
Number of reflections	8686	873
<I/σ(I)>	18.16
Completeness [%]	95.7
Redundancy	3.4
CC(1/2)	0.423	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	30%(w/v) PEG 1500, 40% 1,2-Butanediol