6L1P

Crystal structure of PHF20L1 in complex with Hit 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	51.801, 52.574, 101.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.899 - 1.231
R-factor	0.1844
Rwork	0.184
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sd4
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.900	1.280
High resolution limit [Å]	1.230	1.230
Rmerge	0.064	0.251
Number of reflections	80879	7954
<I/σ(I)>	33.5	7.1
Completeness [%]	100.0
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6M magnesium sulfate, 0.1mM MES, PH 6.5