6L1O
Product bound BacF structure from Bacillus subtillis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.899, 59.190, 85.810 |
| Unit cell angles | 90.00, 103.39, 90.00 |
Refinement procedure
| Resolution | 83.480 - 1.900 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o1b |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.420 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.480 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 54060 | 5503 |
| <I/σ(I)> | 8.24 | |
| Completeness [%] | 94.7 | |
| Redundancy | 3 | |
| CC(1/2) | 0.997 | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 0.1M sodium acetate trihydrate, 0.1M cacodylate trihydrate (pH= 6.5), 30% w/v polyethylene glycol |
