6L1I
Crystal Structure Of of PHF20L1 Tudor1 Y24W/Y29W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 |
| Unit cell lengths | 63.124, 63.124, 26.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.333 - 1.849 |
| R-factor | 0.1643 |
| Rwork | 0.160 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sd4 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.340 | 1.880 |
| High resolution limit [Å] | 1.849 | 1.850 |
| Rmerge | 0.101 | 0.696 |
| Number of reflections | 5374 | 500 |
| <I/σ(I)> | 25 | 2.7 |
| Completeness [%] | 99.5 | |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 4000, 0.2M Lithium Sulfate, 0.1M Tris, PH 8.0 |
