6L1H
Crystal structure of light-dependent protochlorophyllide oxidoreductase from Thermosynechococcus elongatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.899, 96.200, 141.747 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.560 - 2.409 |
R-factor | 0.1827 |
Rwork | 0.181 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rd5 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.783 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.112 | 0.547 |
Rpim | 0.036 | 0.183 |
Number of reflections | 27727 | 2401 |
<I/σ(I)> | 18.1 | |
Completeness [%] | 96.9 | 85.6 |
Redundancy | 11 | 4.5 |
CC(1/2) | 0.988 | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M HEPES, pH 7.5, 20% (w/v) PEG 4,000, 10% (w/v) isopropanol |