6L0V
Structure of RLD2 BRX domain bound to LZY3 CCL motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.673, 31.237, 128.871 |
| Unit cell angles | 90.00, 91.54, 90.00 |
Refinement procedure
| Resolution | 42.942 - 1.347 |
| R-factor | 0.1781 |
| Rwork | 0.177 |
| R-free | 0.20070 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.347 | 1.347 |
| Rmerge | 0.065 | 0.191 |
| Number of reflections | 75903 | 3518 |
| <I/σ(I)> | 26.1 | 3.2 |
| Completeness [%] | 95.4 | 90.6 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | PEG 4000, sodium acetate, Tris-HCl buffer (pH 7.0) |
