6L0I
Crystal structure of dihydroorotase in complex with malate at pH6.5 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.373, 88.471, 103.502 |
| Unit cell angles | 90.00, 95.43, 90.00 |
Refinement procedure
| Resolution | 37.215 - 2.200 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6l0a |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.215 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 76236 | 6417 |
| <I/σ(I)> | 21.1 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.935 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100mM imidazole malate pH 6.5, 18% PEG 4000 |
