6L0A

Crystal structure of dihydroorotase in complex with malate at pH7 from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	85.568, 88.544, 103.079
Unit cell angles	90.00, 95.73, 90.00

Refinement procedure

Resolution	40.823 - 1.790
R-factor	0.1793
Rwork	0.178
R-free	0.20690
Structure solution method	SAD
Data reduction software	Epinorm
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.790	1.790
Number of reflections	143268	14016
<I/σ(I)>	24.7
Completeness [%]	99.5
Redundancy	4.1
CC(1/2)	0.976	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM imidazole malate pH 7, 16% PEG 4000