6L07

Crystal structure of Escherichia coli phosphatidylserine decarboxylase (PE-bound form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	218.191, 218.191, 143.798
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.244 - 3.600
R-factor	0.2434
Rwork	0.241
R-free	0.28910
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.820
High resolution limit [Å]	3.600	3.600
Rmeas	0.084	1.136
Number of reflections	45175	14239
<I/σ(I)>	12.3	1.47
Completeness [%]	99.8	99.5
Redundancy	5.38	5.19
CC(1/2)	0.999	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	pentaerythritol ethoxylate, ammonium sulfate