6L06

Crystal structure of Escherichia coli phosphatidylserine decarboxylase (apo-form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	156.882, 172.012, 80.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.060 - 2.600
R-factor	0.2332
Rwork	0.231
R-free	0.27570
Structure solution method	SAD
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.600	2.600
Rmeas	0.077	1.009
Number of reflections	67495	20374
<I/σ(I)>	11.35	1.03
Completeness [%]	99.5	97.6
Redundancy	3.58	3.6
CC(1/2)	0.998	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Tacsimate