6L02

Crystal Structure of sfYFP66BPAC203Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97852
Spacegroup name	P 41 21 2
Unit cell lengths	51.604, 51.604, 179.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.518 - 1.799
R-factor	0.1746
Rwork	0.173
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yr3
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.863
High resolution limit [Å]	1.799	1.799
Rmerge	0.027	0.180
Rmeas	0.038	0.255
Rpim	0.027	0.180
Number of reflections	23452	2297
<I/σ(I)>	21.84
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.997	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	289	PEG 3350, sodium malonate