6L00

Crystal structure of the cell-wall binding domain (CBD) of endolysin LysIME-EF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	I 4 2 2
Unit cell lengths	59.880, 59.880, 87.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.342 - 1.750
R-factor	0.2251
Rwork	0.223
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ist
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.330	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.089
Number of reflections	8236	552
<I/σ(I)>	2.3
Completeness [%]	99.0
Redundancy	9.4
CC(1/2)	0.999	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	289	20% PEG 4000, 0.2 M NaCl