6KZX
Crystal structure of E.coli DNA gyrase B in complex with 2-oxo-1,2-dihydroquinoline derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-01-15 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.870, 68.568, 68.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.570 - 2.100 |
R-factor | 0.20169 |
Rwork | 0.198 |
R-free | 0.26987 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aj6 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.570 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.094 | 0.335 |
Number of reflections | 10915 | 1072 |
<I/σ(I)> | 9.7 | |
Completeness [%] | 99.7 | |
Redundancy | 4.77 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | MES, PEG200 |