6KZB
Transglutaminase2 complexed with calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2011-10-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 133.080, 216.307, 166.263 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.585 - 3.550 |
| Rwork | 0.227 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pyg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.676 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.585 | 3.620 |
| High resolution limit [Å] | 3.550 | 3.560 |
| Number of reflections | 29265 | 1454 |
| <I/σ(I)> | 8.95 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Potassium chloride, Magnesium acetate tetrahydrate, Sodium cacodylate, Polyethylene glycol 8000 |
