6KZA
Crystal structure of the complex of the interaction domains of E. coli DnaB helicase and DnaC helicase loader
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 121.560, 121.560, 105.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 3.100 |
| R-factor | 0.2337 |
| Rwork | 0.232 |
| R-free | 0.26740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.829 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.980 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmeas | 0.061 | 0.724 |
| Number of reflections | 14617 | |
| <I/σ(I)> | 31.7 | |
| Completeness [%] | 99.3 | |
| Redundancy | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 0.1 M HEPES buffer, pH 7.3, 23% (w/v) PEG 3350 |
