6KZ7

The crystal structure of BAF155 SWIRM domain and N-terminal elongated hSNF5 RPT1 domain complex: Chromatin remodeling complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2011-12-31
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	77.232, 77.232, 207.242
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.100 - 2.280
R-factor	0.1695
Rwork	0.166
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.100	2.343
High resolution limit [Å]	2.280	2.280
Rmerge	0.085	0.405
Number of reflections	20987	2069
<I/σ(I)>	20.16	3.01
Completeness [%]	99.9	92
Redundancy	5.43	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		288	TACSIMATE, HEPES, PEC3350, MICROBATCH, TEMPERATURE 288K