6KYZ

HRV14 3C in complex with single chain antibody YDF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9776
Spacegroup name	P 21 21 2
Unit cell lengths	107.335, 120.846, 71.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.048 - 1.845
R-factor	0.1761
Rwork	0.174
R-free	0.20810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2in2 2ghw
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.845	1.850
Rmerge	0.174	0.493
Rmeas	0.182	0.528
Rpim	0.051	0.182
Number of reflections	80114	2300
<I/σ(I)>	20	2.8
Completeness [%]	99.6	95.4
Redundancy	12.4	7.8
CC(1/2)	0.983	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M citrate 40% PEG600