6KYX

Crystal Structure of Staphylococcus aureus response regulator ArlR DNA binding domain with His tag cleaved

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 64 2 2
Unit cell lengths	68.060, 68.060, 152.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.471 - 2.005
R-factor	0.2046
Rwork	0.200
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.471	2.080
High resolution limit [Å]	2.000	2.000
Rmerge	0.093	1.180
Number of reflections	14804	2282
<I/σ(I)>	21.05
Completeness [%]	99.7
Redundancy	24.9
CC(1/2)	0.999	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	3.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6