6KYQ

Crystal structure of DCLK1 Autoinhibited Kinase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	77.600, 77.522, 178.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.287 - 2.141
R-factor	0.1694
Rwork	0.161
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fg9
RMSD bond length	0.002
RMSD bond angle	0.583
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.141	2.150
Number of reflections	59632	2916
<I/σ(I)>	18.9
Completeness [%]	100.0
Redundancy	6.5
CC(1/2)		0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6M NaH2PO4, 1.6M K2HPO4, PH7.8