6KYB
Crystal structure of Atg18 from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-09 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 |
Unit cell lengths | 95.330, 95.330, 300.871 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.628 - 2.800 |
R-factor | 0.2168 |
Rwork | 0.214 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4exv |
RMSD bond length | 0.007 |
RMSD bond angle | 1.295 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.157 | 0.113 | 1.706 |
Rmeas | 0.163 | 0.119 | 1.770 |
Rpim | 0.043 | 0.033 | 0.462 |
Total number of observations | 578363 | ||
Number of reflections | 39054 | 2032 | 1906 |
<I/σ(I)> | 4.8 | ||
Completeness [%] | 98.3 | 99.9 | 97 |
Redundancy | 14.8 | 14.9 | 13.4 |
CC(1/2) | 0.983 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M Hepes7.5, 10%PEG6000, 5%MPD |