6KY9

Crystal structure of ASFV dUTPase and UMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-05-12
Detector	RDI CMOS_8M
Wavelength(s)	1.03923
Spacegroup name	P 43
Unit cell lengths	57.418, 57.418, 149.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.640 - 1.700
R-factor	0.1897
Rwork	0.188
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.696	1.700
Rpim	0.030	0.026
Number of reflections	53337	5306
<I/σ(I)>	24.81	3.07
Completeness [%]	100.0
Redundancy	13.8
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Citric acid pH 3.5, 14% w/v Polyethylene glycol 1,000.