6KY8

Crystal structure of ASFV dUTPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-07
Detector	RDI CMOS_8M
Wavelength(s)	1.03923
Spacegroup name	I 21 3
Unit cell lengths	97.855, 97.855, 97.855
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.949 - 2.301
R-factor	0.2236
Rwork	0.222
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	7082	690
<I/σ(I)>	53.9
Completeness [%]	100.0
Redundancy	38.6
CC(1/2)	1.000	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M ammonium citrate tribasic pH 7.0, 12% w/v Polyethylene glycol 3,350