6KXE
The ishigamide ketosynthase/chain length factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-09-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.426, 69.426, 265.465 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.780 - 1.810 |
| R-factor | 0.1656 |
| Rwork | 0.163 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.630 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.780 | 1.850 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.163 | 2.012 |
| Rmeas | 0.168 | 2.075 |
| Rpim | 0.052 | 0.698 |
| Number of reflections | 65008 | 3883 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 19.6 | |
| CC(1/2) | 0.999 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.1 M sodium phosphate monobasic, 1.65 M potassium phosphate dibasic, and 0.1 M sodium acetate/acetic acid buffer |
