6KX5
Crystal structure of mouse Cryptochrome 1 in complex with KL044 compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-04 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.410, 54.400, 61.420 |
| Unit cell angles | 63.90, 88.99, 83.65 |
Refinement procedure
| Resolution | 35.040 - 2.000 |
| R-factor | 0.1793 |
| Rwork | 0.176 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kx4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.868 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.040 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.518 | |
| Rmeas | 0.266 | |
| Rpim | 0.134 | |
| Number of reflections | 34078 | 4936 |
| <I/σ(I)> | 4.6 | 2.4 |
| Completeness [%] | 98.3 | |
| Redundancy | 4 | |
| CC(1/2) | 0.930 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% w/v PEG 3350, 3% v/v ethylene glycol, 200 mM NH4Cl |
