6KX4
Crystal structure of mouse Cryptochrome 1 apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-04 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.930, 54.860, 60.950 |
| Unit cell angles | 63.79, 89.11, 83.84 |
Refinement procedure
| Resolution | 31.290 - 2.000 |
| R-factor | 0.1781 |
| Rwork | 0.175 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k0r |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.832 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.290 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.320 | |
| Number of reflections | 33688 | 4896 |
| <I/σ(I)> | 4.2 | 2.9 |
| Completeness [%] | 98.3 | 97.5 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.800 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES-NaOH pH 6.5, 15% (w/v) PEG3350 and 10 mM EDTA |
