6KWW
HslU from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 146.618, 189.602, 215.813 |
| Unit cell angles | 90.00, 92.62, 90.00 |
Refinement procedure
| Resolution | 36.370 - 3.000 |
| R-factor | 0.2353 |
| Rwork | 0.235 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g3i |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.089 | 0.977 |
| Rpim | 0.035 | 0.393 |
| Number of reflections | 215969 | 10127 |
| <I/σ(I)> | 19.06 | |
| Completeness [%] | 93.1 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.998 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | Ammonium sulfate, Bis-tris:Hcl, penthaerythritol ethoxylate (15/4_ EO/OH) |
