6KWV

A Crystal Structure of OspA mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	34.437, 53.348, 65.501
Unit cell angles	90.00, 98.82, 90.00

Refinement procedure

Resolution	18.430 - 1.370
R-factor	0.192
Rwork	0.191
R-free	0.20930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g8c
RMSD bond length	0.004
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.370	1.370
Rmerge	0.052	0.177
Number of reflections	48779	2469
<I/σ(I)>	28.2	8.94
Completeness [%]	99.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	282	38% PEG 400, 0.1M Tris pH 8.0, 15.4mg/mL