6KWU
A Crystal Structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.081, 54.388, 66.236 |
| Unit cell angles | 90.00, 100.18, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.430 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.726 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.093 | 0.344 |
| Number of reflections | 42482 | 2139 |
| <I/σ(I)> | 20.2 | 7.27 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 282 | 36% PEG 400, 0.1M Tris pH 7.0, 0.05M MgSO4, 24.6 mg/mL (12.3 mg/mL 2uL:1uL) |
